골막기원세포의 조골세포 분화과정에서 나타나는 혈관내피전구세포의 증식
PROLIFERATION OF ENDOTHELIAL PROGENITOR CELLS BY OSTEOGENIC DIFFERENTIATION OF PERIOSTEAL-DERIVED CELLS
김종렬, 송정호, 김욱규, 박봉욱, 하영술, 김진현, 김덕룡, 조영철, 성일용, 변준호,
소속 상세정보
김종렬 ( Kim Jong-Ryoul ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
송정호 ( Song Jung-Ho ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
김욱규 ( Kim Uk-Kyu ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
박봉욱 ( Park Bong-Wook ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
하영술 ( Hah Young-Sool ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
김진현 ( Kim Jin-Hyun ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
김덕룡 ( Kim Deok-Ryong ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
조영철 ( Cho Yeong-Cheol ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
성일용 ( Sung Iel-Yong ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
변준호 ( Byun June-Ho ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
KMID : 0355620090350040205
Abstract
Purpose: The purpose of this study was to examine the expression of various angiogenic factors during osteoblastic differentiation of periostealderived cells and the effects of osteogenic inductive medium of periosteal-derived cells on the proliferation of endothelial progenitor cells.
Materials and Methods : Periosteal-derived cells were obtained from mandibular periosteums and introduced into the cell culture. After passage 3, the cells were divided into two groups and cultured for 21 days. In one group, the cells were cultured in the DMEM supplemented with osteogenic inductive agent, including 50g/ml L-ascorbic acid 2-phosphate, 10 nM dexamethasone and 10 mM -glycerophosphate. In the other group, they were cultured in DMEM supplemented without osteogenic inductive agent. VEGF isoforms, VEGFR-1, VEGFR-2, and neuropilin-1 mRNA expression was observed. Human umbilical cord blood-derived endothelial progenitor cell proliferation was also observed.
Results: The expression of VEGF isoforms was higher in osteogenic inductive medium than in non-osteogenic inductive medium. The expression of VEGFR-2 was also higher in osteogenic inductive medium than in non-osteogenic inductive medium. However, the expression of VEGFR-1 and neuropilin-1 was similar in both osteogenic inductive medium and non-osteogenic inductive medium. In addition, conditioned medium from differentiated periosteal-derived cells stimulated human umbilical cord blood-derived endothelial progenitor cell numbers compared to conditioned medium from non-differentiated periosteal-derived cells.
Conclusion: These results suggest that in vitro osteoblastic differentiation of periosteal-derived cells has angiogenic capacity to support endothelial progenitor cell numbers.
키워드
Periosteal-derived cells;Angiogenic capacity;Endothelial progenitor cells
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